Use: Tetrathionate Broth Base with iodine-iodide solution added, is a selective enrichment liquid medium used for the cultivation of Salmonella typhi from feces, urine, foods and other materials of sanitary importance. Description: The use of Tetrathionate Broth for the cultivation of Salmonella species was first described by Mueller. Mueller demonstrated that lactose fermenting members of the Enterobacteriaceae were inhibited or killed in the medium while the salmonellae grew almost unrestricted. Shaeffer demonstrated the enrichment properties of the medium, reporting that he obtained four times as many isolates of salmonellae after specimen enrichment in Tetrathionate Broth than found on direct platings. Kauffman modified the formula and reported marked increase in recovery of Salmonella. Tetrathionate Broth Base conforms to the formulation recommended by the American Public Health Association for use in the examination of dairy productss and foods for the presence of salmonellae. Tetrathionate Broth Base is enriched with iodine solution for the selective cultivation of Salmonella species. Bile salts are incorporated in the medium as the selective agent to retard the growth of gram- negative bacilli other than salmonellae, and inhibit gram-positive organisms.
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